Objectives

• Define PCR, TMA, SDA, LAMP, and LCR

• Recognize the requirements for PCR to succeed

• Troubleshoot common qPCR issues

• Recall the basic steps of PCR

• Define many of the important PCR controls

• Recall some clinical applications for PCR

To Do List:

• Take the pre-lesson assessment

• Watch summary lecture video

• Watch PCR-specific videos

• Watch TMA video

• Watch LAMP video

• Watch SDA video

• Download and review PCR handout

• Complete post-lesson assessment

Complete Pre-Lesson Assessment

Target Amplification Lecture by Cullen Lilley

PCR Technologies

Digital PCR (dPCR) Theory

PCR Quality Control

qPCR Controls (Stop watching after 15:00)

Loop-Mediated Amplification

Transcription Mediated Amplification

Strand Displacement Amplification

Download and review PCR review sheet

• Download the PCR review sheet HERE

Congratulations on finishing this module! In order to get your course certificate, please complete the exam and satisfaction survey linked below.

The exam will consist of 10 questions with a short feedback section at the beginning. You will have TWO attempts to take the quiz and pass with a score of 60% or higher. If you do not receive your certificate with 10 minutes of completing your exam, please contact us.