Molecular Microbiology Lesson 2:

Signal Amplification, FISH, and MALDI-TOF


Objectives

  • Define PCR, TMA, SDA, LAMP, and LCR

  • Recognize the requirements for PCR to succeed

  • Troubleshoot common qPCR issues

  • Recall the basic steps of PCR

  • Define many of the important PCR controls

  • Recall some clinical applications for PCR


To Do List:

  • Take the pre-lesson assessment

  • Watch summary lecture video

  • Watch bDNA theory video

  • Watch hybrid capture theory video

  • Watch PNA FISH procedure video

  • Watch MALDI-TOF theory video

  • Complete post-lesson assessment


Complete pre-lesson assessment


Signal Amplification Lecture by Cullen Lilley


bDNA Assay and HIV Testing by Dr. Li

Hybrid Capture Theory


PNA FISH Procedure


MALDI-TOF Theory


 

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The exam will consist of 11 questions with a short feedback section at the beginning. You will have TWO attempts to take the quiz and pass with a score of 60% or higher. If you do not receive your certificate with 10 minutes of completing your exam, please contact us.