Objectives

• Describe the theory behind the various sequencing technologies

• Differentiate between the major sequencing technologies

• Determine the proper sequencing technology for a given project

• Understand basic phylogenetic trees (more in the next lesson)

• Analyze molecular epidemiologic data

To Do List:

• Take the pre-lesson assessment

• Watch summary lecture video

• Watch NGS overview video video

• Watch Sanger sequencing video

• Watch Pyrosequencing video

• Watch IonTorrent video

• Watch Illumina video

• Watch PacBio video

• Watch NanoPore video

• Complete post-lesson assessment

Complete Pre-Lesson Assessment

Signal Amplification Lecture by Cullen Lilley

NGS Overview by Dr. Chow from iBiology

Sanger Sequencing

Pyrosequencing Video

IonTorrent Video

Illumina Overview

PacBio Sequencing

NanoPore Sequencing

Molecular Epidemiology Lecture

Listen to this Episode of Meet the Microbiologist

(Required Info for Exam)

Congratulations on finishing this module! In order to get your course certificate, please complete the exam and satisfaction survey linked below.

The exam will consist of 10 questions with a short feedback section at the beginning. You will have TWO attempts to take the quiz and pass with a score of 60% or higher. If you do not receive your certificate with 10 minutes of completing your exam, please contact us.